Fig. 3
From: Nano-Pulse Stimulation is a physical modality that can trigger immunogenic tumor cell death
PE Annexin V and 7-AAD labeling to determine stage of apoptotic cell death at 3 h and 24 h. The percent of cells at different stages is indicated by color. Gray bars represent live viable cells; Blue bars represent cells in the early stages of apoptosis (PE Annexin V+/7’AAD-); Orange bars represent cells in the later stages of apoptosis (PE Annexin V+/7’AAD+); Yellow bars represent cells in the latest stages of cell death (PE Annexin V-/7-AAD+). The number of pulses applied to achieve the indicated J/ml for all cell lines are indicated above the MCA205 plot. Significant difference from untreated controls by one-way ANOVA with between group analysis performed using the Dunnett’s test. *p < 0.05; **p < 0.01