Fig. 2
From: Vaccination with inhibin-α provides effective immunotherapy against testicular stromal cell tumors
Vaccination against rmInα inhibits growth of transplantable TSC tumors. Significant inhibition of transplantable tumor growth occurred when rmInα vaccination occurred (a) prophylactically 8 days prior to inoculation with 1-10 TSC tumor cells or (b) as treatment of palpable I-10 TSC tumors. (c) Immunohistochemical analysis showed virtually no CD3+ T cells in I-10 TSC tumors 32 days after vaccination with CFA alone whereas (d) extensive CD3+ T cell infiltrates were evident in I-10 tumors taken from mice 32 days after vaccination against rmInα. Arrows point to examples of CD3+ T cells. (e) Flow cytometry analysis showed that 28.3% of the TILs were CD3 + CD4+ T cells (upper left panel) and 57.9% of these CD4+ T cells expressed the CD44 activation marker (upper right panel). Only 15% of TILs expressed the CD3 + CD8+ phenotype (lower left panel) and only 66.1% of these CD8+ T cells expressed the CD44 activation marker (lower right panel). Error bars indicate ± SE, and asterisks indicate significance