Fig. 3

Western blot of CAR T proteins in primary human T cells. Cell lysates prepared from human primary T cells transduced with single (19B, 19A, 20A) or tandem-CAR19 and CAR20 (1920, 2019) constructs were resolved on a gradient (4–12%) SDS-PAGE gel and probed with an antibody against CD3-zeta. T cells from the same donor transduced with a lentiviral vector encoding green fluorescent protein (GFP), or non-transduced T cells (N.T.) served as controls. Molecular weight markers, in KDa, are listed to the right