Fig. 2

GITRL-FP PD effects on T cells in CT26 tumor–bearing mice. a Tumor volumes were measured after IP doses of untreated[solid circle●] (controls) or GITRL-FP[solid square■] at 1 mg/kg biweekly for 4 total doses. On day 18 mice were sacrificed to examine (b) Tregs and (c) CD8 T cells. d Single-cell suspensions of spleens and tumors were restimulated with AH1 peptide/protein transport inhibitor and stained for IFNγ and TNFα. Proliferation was measured by KI-67 on (e) CD4 T cells and (f) CD8 T cells