Fig. 3

Levels of GITRL-FP bound to T-cell populations in mice receiving 3 doses of biotinylated isotype antibody or biotinylated GITRL-FP on days 11, 14, and 18. GITRL-FP drug levels were detected using SA-APC and free GITR was detected using DTA-1 anti-GITR antibody on a) Spleen CD4 b) Spleen CD8 c) Tumor CD4 d) Tumor CD8