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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Adoptive cell therapy using PD-1+ myeloma-reactive T cells eliminates established myeloma in mice

Fig. 2

PD-1+ T cells from myeloma-bearing mice are phenotypically heterogeneous and secrete effector cytokines. KaLwRij mice were inoculated with 2 × 106 5T33-GFP cells iv. Spleens were harvested 28 days later for analysis. a Flow cytometric analysis of PD-1 co-expression with inhibitory receptors TIM-3 and LAG-3, and activation markers CD69, CD137, OX-40 and CD103 on CD8+ and CD4+ T cells. b Percentages of PD-1+Foxp3+CD4+ T cells analyzed by flow cytometry. c T cells were activated with 1 μg/ml plate bound anti-CD3 and anti-CD28 for 6 h, and analyzed for the presence of intracellular cytokines by flow cytometry. d Multiplex cytokine analysis of culture supernatants from T cells activated with 5 μg/ml plate bound anti-CD3 (clone 2C11) for 48 h. Data shown are representative of more than four independent analyses. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001

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