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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Pretreatment antigen-specific immunity and regulation - association with subsequent immune response to anti-tumor DNA vaccination

Fig. 4

Prostate cancer patients had preexisting immune responses to the PAP protein. PBMCs from responder or non-responder patients were stimulated 1 or 3 day(s) with PAP, PSA, AR LBD (AR), and Con A. Supernatants from cultures were collected and analyzed for cytokine concentrations (a) Granzyme B, (b) IFNγ, (c) IL-2, (d) IL-4, (e) IL-10, (f) IL-6, and (g) IL-17a using a cytokine bead array. Each dot represents the cytokine expression level for an individual prostate cancer patient (closed squares-responders (n = 7) and open square-non-responders (n = 23)). Samples were analyzed in triplicates and averaged. Lines represent median values for each data set. Statistical comparisons were made using a Mann Whitney t test. As in Fig. 3, cryopreserved samples were not available for 8 subjects

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