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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Pretreatment antigen-specific immunity and regulation - association with subsequent immune response to anti-tumor DNA vaccination

Fig. 5

Patients that were long term immune responders had preexisting PAP-specific regulatory responses. Pretreatment PBMCs from immune responder (n = 9) and non-responder patients (n = 23) were injected into the footpads of SCID mice with TT/D (recall antigen) alone or in combination with PAP or PSA. Each dot represents the net swelling (10−4 in.) after 24 h. This was defined as the antigen-specific swelling measurement minus the swelling due to the PBMCs and PBS alone. Shown is the net DTH immune response (10−4 in.) to TT/D alone (closed circles, responders; closed squares, non-responders) or in combination with PAP (a) or PSA (b) (circles, immune responders; squares, immune non-responders) for each patient. Statistical comparisons were made using a paired, non-parametric t test analysis. The log-transformed fold change is shown comparing c) TT/D PAP to TT/D and d) TT/D PSA to TT/D for immune responders and non-responders. Comparison of fold change was made using a Mann Whitney t test

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