Fig. 3

Six days after the second Listeria-based immunotherapy treatment, mice were euthanized and tumors harvested and profiled for a. total number of CD8⁺ T cells; and b. total number of E7-specific CD8⁺ T cells (CD8⁺E7⁺) per 1e⁶ tumor cells. Ratios for CD8⁺/Tregs (c) and CD8⁺E7⁺/Tregs (d) were calculated. e. Six days after the second treatment with Listeria-based immunotherapy treatment, mice were euthanized and spleens were collected for ELISPOT analysis. Antigen-specific CD8⁺ T cells in the presence of E7_49–57 peptide versus irrelevant peptide control was assayed by ELISPOT. Values are presented as number of spots from E7_49–57 restimulated culture minus irrelevant Ag re-stimulated culture per million splenocytes. (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001). Experiment was repeated twice with n = 5/group with similar results