Fig. 1

Selumetinib alters the phenotype of antigen presenting cells and suppresses T-cell activation in vitro. a Human PBMCs stimulated with SEA, anti-CD3 antibody and either 30 μg/ml of tremelimumab or isotype control, were incubated with increasing concentrations of selumetinib for 72 h. Levels of IL-2 in supernatants were determined by immuno-assay. Data presented as mean (± SEM) of triplicates. b Flow cytometry analysis of mouse CT26 tumor cells following 48 h treatment with selumetinib or DMSO vehicle control and stained for H2-Kd and PD-L1. c Flow cytometry analysis of human monocyte-derived dendritic cells after 8 days in culture with GM-CSF and IL-4. Cells were either untreated or activated with CD40L and treated with selumetinib or DMSO vehicle control for the last 48 h of culture. Histograms for staining with specific antibodies for mDCs activated with CD40L and treated with DMSO vehicle (solid line); or mDCs left untreated (dashed line); and isotype control staining of untreated mDCs (filled). Percentage of gated cells are shown in histograms for the CD40L-activated + DMSO condition. d The percentages of CD80⁺, CD83⁺, CD86^high and HLA-DR^high cells of total live cells, and frequency of live cells out of total cells are shown. Plotted data are single measurements