Fig. 6

CD8⁺ T cells can uptake tumor-derived exosomes. 3 μg (1.5 μg/ml) of Tu167 TDEs were labeled with PKH67 dye. The flow-through produced during the concentration of the labeled TDEs, and the labeled TDEs were incubated with 1 × 10⁶ CD3⁺CD8⁺ T cells for 30 and 120 min. Unbound exosomes were removed from cells by multiple washes with flow cytometry buffer (1× PBS, 2 mM EDTA, and 0.5% BSA). a. Dot plots above were first gated off of CD3⁺ T cells and then gates were set to CD8⁺ T cells vs. PKH67 negative (T cells not incubated with labeled TDEs). b. Cells positive for TDE uptake are characterized as being double positive for CD8⁺ and PKH67. Data represents the mean ± SD of 3 replicates