Fig. 1

Chimaeric RHDV VLP Containing CRC TAAs. Each chimaeric RHDV VLP construct was expressed in Sf21 cells and purified for analysis and applications in vaccination. a Diagram outlining the structure of the recombinant VP60 produced for each chimaeric VLP construct, including non-mutant H-2K^b restricted peptide sequences, ALL linker and GGS spacer sequence. b Western blot probing for RHDV VP60 with T.VP60, S.VP60 and TS.VP60 represented in comparison to unmodified VP60. Electron microscopy was used to identify the formation of intact VLP from the expression of c T.VP60, d S.VP60 and e TS.VP60