Fig. 2
From: Multi-target chimaeric VLP as a therapeutic vaccine in a model of colorectal cancer
Induction of Target-specific Immunity with Chimaeric VLP. The ability of each chimaeric VLP construct to induce an immune response specifically targeting their incorporated epitope was assessed using a modified in vivo cytotoxicity assay. a Modified in vivo cytotoxicity protocol, using a combination of CFSE and VPD staining to simultaneously monitor cytotoxicity against multiple target populations. Responses were assessed for T.VP60, S.VP60 and TS.VP60 in comparison to unmodified VP60 or synthetic peptides, calculating specific lysis of the b topIIα, c survivin and d topIIα/survivin target populations. Results are representative of three independent repeats. Statistical analysis performed using unpaired t-tests. NS = Non-significant, *** p < 0.001, **** p < 0.0001