Fig. 4

Calcium mobilization in transduced NK cells. a Multiplex analysis of CXCR2 ligands in the supernatants of the RCC cell lines ACHN, 786-O, MAR, CAKI-2, and A498. CXCL1 in ACHN cells was above the quantification limit of 13,990 pg/mL, CXCL5 in A498 cells was below the quantification limit of 603 pg/mL. b Calcium mobilization in CXCR2- and NGFR-transduced NK cells stimulated with recombinant CXCL8 (50 ng/mL) or supernatant from the RCC cell lines ACHN, MAR or A498. Values are Fluo-3 relative fluorescent units (RFU) normalized to the baseline prior to the addition of stimuli. c Calcium response in CXCR2- and NGFR-transduced NK cells after addition of the stimuli calculated as the normalized area under curve (AUC)