Fig. 5

CXCR2-specific migration of CXCR2-transduced NK cells. a Time course of the migration of non-transduced (NT), NGFR- or CXCR2-transduced NK cells toward a pool of recombinant CXCL1, CXCL2, CXCL3, and CXCL8. Cell migration was analyzed with the IncuCyte ZOOM live cell imager and expressed as area occupied by NK cells on the top surface normalized to the initial top value (n = 4 per condition). Repeated measures two-way ANOVA was applied to analyze data. b Transwell migration assay of NT, NGFR- or CXCR2-transduced NK cells toward a pool of the recombinant CXCR2 ligands CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8. The number of migrated cells was determined by automatic counting using a flow cytometer from three technical replicates per experiment (n = 7). c NK cell-mediated cytotoxicity against K562 cells after transwell migration toward medium with or without recombinant CXCR2 ligands (n = 3). d Equal concentrations of CXCR2 ligands were added to the upper and lower chambers of a transwell assay of NGFR- and CXCR2-transduced NK cells. Results are representative of three experiments with two technical replicates. e Transwell assay of NGFR- and CXCR2-transduced NK cells toward recombinant CXCL1, CXCL8 and CXCL5 following pre-incubation with the selective CXCR2 inhibitor SB225002. Results are representative of three experiments for CXCL1 and CXCL8 and of two experiments for CXCL5 with two technical replicates. f Transwell assay of NGFR- and CXCR2-transduced NK cells toward supernatants from RCC cell lines. Data are mean values from three technical replicates per experiment (n = 4 for ACHN, 786-O, and MAR; n = 3 for Caki-2 and A498). Percent migrated cells are calculated based on total cell input. *, P < 0.05, **, P < 0.01 and ****, P < 0.0001