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Fig. 3 (abstract P385). | Journal for ImmunoTherapy of Cancer

Fig. 3 (abstract P385).

From: 32nd Annual Meeting and Pre-Conference Programs of the Society for Immunotherapy of Cancer (SITC 2017): Part Two

Fig. 3 (abstract P385).

The Wnt5a-CXCL5 Pathway Drives Gr-MDSC Recruitment in Response to anti-PD-1 Ab Therapy and Promotes the Development of anti-PD-1 Ab Adaptive Resistance. A. Flow cytometry analysis of Gr-MDSCs in Wnt5a-silenced BRAF(V600E)-PTEN-/- melanomas. B. Immunohistochemistry of Gr-MDSCS in Wnt5a-silenced BRAF(V600E)-PTEN-/- melanomas. C. Flow cytometry analysis demonstrates that Gr-MDSC recruitment in response to anti-PD-1 ab therapy is ablated in Wnt5a-silenced BRAF(V600E)-PTEN-/- melanomas. Representative flow cytometry dot plots provided. D. IFNg ELISPOT and tissue immunofluorescence show that melanoma Wnt5a silencing enhances the activation of TRP2-specific CD8+ T cells and promotes the expression of PD-L1. Representative images provided on the left. E. Wnt5a silencing sensitizes BRAF(V600E)-PTEN-/- melanomas to anti-PD-1 ab therapy. F. CXCL5 silencing suppresses Gr-MDSC recruitment and sensitizes BRAF(V600E)-PTEN-/- melanomas to anti-PD-1 ab therapy. Representative flow cytometry dot plots shown. All data is mean +/- SEM. *P<0.05

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