Fig. 1 (abstract P389).

Aurora kinase inhibitors were identified to enhance T cell mediated cytotoxicity. (A) Patient derived melanoma tumor cells were incubated with one of 850 different compounds per well in triplicate in a high-throughput screen. After 24 hours, the compound was washed off and the tumor cells were co-cultured with autologous TILs for three hours, followed by intracellular staining for cleaved caspase-3. The percentage caspase-3 positive tumor cells is used as a read-out for the amount of apoptosis among DDAO stained tumor cells. (B) The comboscore is calculated to assess the enhanced cytotoxicity of combined compound and TIL treatment over either treatment alone. (C) Mel2549 cells were treated with 2uM AZD1152 or DMSO, TIL at a 3:1 E:T ratio or the combination of AZD1152 and TIL. Four other patient derived melanoma cell lines showed similar enhanced cytotoxicity for the combination treatment