Fig. 2

CAR-T cells show high polyfunctionality in anti-tumor effector and stimulatory functions. a Polyfunctional breakdown of CD3+, CD4+ and CD8+ T cells at the single-cell level across 4 donors. The T cells of donors 1, 3 and 4 show a 6 to 47-fold increase in overall polyfunctionality when stimulated with anti-CAR beads, compared to IgG stimulation. By comparison, donor 2 only shows a 2-fold increase. b Polyfunctional strength index (PSI) computed for CD3+, CD4+, and CD8+ T cells at the single-cell level across 4 donors. The profiles of donors 1, 3 and 4 is dominated by effector and stimulatory cytokine subsets. c Heat map and dendrogram visualization applied to the CD3 T cell secretion data. There is one heat map per donor, and each column corresponds to a single cytokine, while rows correspond to individual cells. Non-secreting cells are excluded from the heat maps. The colors indicate log transformed secretion intensities (red = low, green = high). At a high level, this visualization illustrates some differences across donors and which cytokines are commonly secreted in tandem. However, the clustering is done individually per donor, and it is difficult to map clusters to functional subsets