Fig. 3

Image analysis of CD8+ TILs in PD-L1–positive and –negative tumor. Serial sections of tumor specimens of 24 non-enrolled NSCLC patients of clinical trial NCT01693562 were immunostained for CD8 alone (a, b, c) and with a CD8/PD-L1 dual immunostain (d, e, f). CD8+ TILs were immunolabeled brown in the mono stain (a) and purple in the dual stain (d), with PD-L1 labeled brown. IA detected CD8+ TILs as blue in the mono stain (b) and lavender in the dual stain (e). IA further classified tumor cells (c, yellow areas) in the mono stain or PD-L1+ cells (f, red areas) in the dual stain; darker shades of red represent more intense PD-L1 expression. IA determined the numbers of CD8+ TILs in the two stains to be comparable (g); Pearson (PCC), Spearman (SCC), and Lin (CCC) concordance values are shown