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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Distinct predictive biomarker candidates for response to anti-CTLA-4 and anti-PD-1 immunotherapy in melanoma patients

Fig. 1

viSNE analysis of CyTOF data results in well-defined immune subset maps in PBMC. Preliminary gating of CyTOF data to define live intact single cells, was performed in CytoBank. Then viSNE mapping of the healthy donor PBMC and patient baseline PBMC are shown. a All ungated events were sequentially gated in Cytobank to identify live intact single cell events as described in Methods. a) 191Ir DNA1 and 140Ce beads were used to identify cells. b) Singlets were identified by gating on cells positive for the DNA markers 191Ir DNA1 and 193Ir DNA2. c) Event Length of singlets from b) was used to obtain intact singlets. d) Live intact singlets were obtained by gating for intact singlets from c) which were negative for dead cell marker 115In maleimide DOTA. Subsequent viSNE analysis was performed using live intact single cell events obtained from d). b viSNE map of healthy donor PBMC showing distinct immune subsets. The heat spectrum associated with each graph indicates the relative expression level of each marker. c Various immune subsets were grouped into distinct islands on the viSNE map

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