Distinct predictive biomarker candidates for response to anti-CTLA-4 and anti-PD-1 immunotherapy in melanoma patients

Table 3 CyTOF panel for the phenotypic and functional analysis of immune cell subsets

A 40-marker CyTOF panel for the phenotypic and functional analysis of immune cell subsets in melanoma patients treated with anti-CTLA-4 or anti-PD-1 therapy is shown. The element and isotope of the metal tag conjugated to each antibody and non-protein subject (italic) is indicated under the ‘Metal Label’ column. ‘Specificity’ indicates the target recognized by the metal-conjugated antibody or non-protein subject. 193Ir/195Ir DNA Intercalator and 115In Maleimide DOTA live/dead stain facilitate the identification of live intact singlets while calibration beads (140Ce) are important for data pre-processing. Antibody clones are listed when applicable