Fig. 2

Sipuleucel-T and DNA immunization elicits PAP-specific cellular immune responses. a ELISPOT was used to determine the frequency of antigen-specific IFNγ-secreting T-cells over multiple time points. Shown are results for one patient (ID004) obtained in real-time at each time point. b ELISPOT was also used to determine the frequency of antigen-specific granzyme B-secreting T-cells over multiple time points for the same individual. Asterisks denote positive immune responses elicited, defined as an antigen-specific response (statistically higher than the media-only control, p < 0.05 by t-test) that was at least 3-fold higher than baseline and with a frequency > 1:100,000 cells. c Shown are the comprehensive summary data from immune monitoring by evaluation for PAP-, PSA-, GM-CSF, or tetanus-specific immune responses as assessed by IFNγ or granzyme B secretion by ELISPOT. A positive immune response (red) was defined as an antigen-specific response (statistically higher than the media-only control, p < 0.05 by t-test) that was at least 3-fold higher than baseline and with a frequency > 1:100,000 cells. Black squares indicate a time point where an assessment was performed, but in which immune response criteria were not met. White squares indicate time points where no data was collected