Skip to main content
Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Complete intracranial response to talimogene laherparepvec (T-Vec), pembrolizumab and whole brain radiotherapy in a patient with melanoma brain metastases refractory to dual checkpoint-inhibition

Fig. 3

Representative tissue analysis using Quantitative Multiplex Immunofluorescence (qmIF). a 4 μm tissue section slide is processed using sequential qmIF protocol which uses tyrosine signal amplification following application of secondary antibody. After tissue processing, multispectral tissue images are obtained. b Cellular phenotyping is performed using companion software (inForm) which uses machine learning to perform automated cell phenotyping based on representative cell selection producing a Cartesian map with the X,Y coordinates of each cell in the imaged tissue, along with its assigned phenotype. c Spatial analysis can be performed using a variety of approaches including nearest neighbor calculation. As an example, distance between tumor cells (SOX10+) and nearest neighboring CTL (CD3 + CD8+) is being depicted

Back to article page