Fig. 6

HER2-CAR T cells administered intrathecally to non-human primates (NHP) did not cause toxicity. a Flow cytometry of truncated HER2 expression (left) and HER2 CAR expression (right) in NHP T cells prior to infusion. The transduction efficiency for truncated HER2 and HER2 CAR ranged from 45 to 90% in all cell cultures. b Flow cytometry of truncated HER2 expression (left) and HER2 CAR expression (right) in T cells from the cerebrospinal fluid (CSF) of NHP ZE32 on Day 1 after infusion. T-HER2 and HER2 CAR T cells were not detected in the other three animals, nor were appreciable levels detected after Day 1 in animal ZE32. c Summary of CSF cytokine levels after infusion of T-HER2 target cells and HER2 CAR T cells. Separate time courses of cytokine measurements, performed by ELISA, are shown for each animal in the experiment. Increases in IL-2 and IL-6 were detected in the CSF of most animals, suggesting antigen recognition of the truncated HER2 protein by the HER2 CAR T cells. IL-8, IL-10, and IL-1β were also tested for, but were undetectable in all samples