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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells

Fig. 2

Human CD46 can be stably expressed on murine cells. a. Clustal Omega protein sequence alignment of murine and human CD46 (GenBank accession BAA31859.1 and BAA12224.1, respectively). The murine and human CD46 protein sequences have a similarity of 49%. Red bold font indicate binding sites of human CD46 and Ad11 fiber knob, as described by Persson et al. [31]. b and c. Murine cells were transduced with a lentiviral vector to generate cell lines stably expressing human CD46. CD46 surface expression in murine and human cell lines was compared by flow cytometry by staining 1 × 106 cells/sample using PE-human CD46 or the corresponding isotype control. Data show (b) the geometric mean of the intensity of CD46 expression and (c) the proportion of cells expressing CD46. Data represent biological triplicates, shown as mean ± SEM. Significance between the parental and corresponding CD46 cell line was assessed using one-way ANOVA with Tukey’s Post Hoc analysis. *, p < 0.05; ***, p < 0.001; ns, not significant

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