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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells

Fig. 3

Expression of human CD46 allows EnAd to undergo replication-independent GFP but not adenovirus major late promoter-driven protein expression. NMuMG, NMuMG-CD46, CT26, CT26-CD46, and A549 cells were seeded at 1 × 104 cells/well in 96-well plates before infection with 5000, 1000, 100, 10, or 1 virus particles/cell (VPC) of either (a) EnAd-CMV-GFP or (b) EnAd-SA-GFP, or mock-infected. Cells were harvested at 5 days post-infection and analysed by flow cytometry for GFP expression. c At 5 days post-infection, the surviving fraction of EnAd-CMV-GFP-infected cells compared to mock-infected was measured by MTS. Data represent means of three independent experiments, shown as mean ± SEM. Significance within each treatment was assessed using two-way ANOVA with Bonferroni correction compared to mock-infected cells. **, p < 0.01; ***, p < 0.001

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