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Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells

Fig. 6

Coinfection with MAV1 fails to complement EnAd replication. a NMuMG, NMuMG-CD46, and A549 or (b) CT26, CT26-CD46, and A549 cells were seeded at 1 × 104 cells/well in a 96-well plate before coinfection with crude MAV1-containing supernatant diluted at 1:2, 1:10, 1:100, or 1:1000 and 5000 virus particles/cell (VPC) of either EnAd-CMV-GFP or EnAd-SA-GFP, or mock-infected. Five days post-infection, GFP expression in cells was analysed by flow cytometry. c NMuMG, NMuMG-CD46, and 293A cells were coinfected with crude MAV1-containing supernatant diluted at 1:2 or 1:1000 and 5000 virus particles/cell of EnAd-CMV-GFP. At 2 and 72 h post-infection, cells were washed and harvested for genomic DNA extraction. EnAd genome copies in 30 ng of genomic DNA were quantified by qPCR using Taqman probes. Data represent biological triplicates, shown as mean ± SEM

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