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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells

Fig. 7

Several MAV1 genes improve CMV promoter-driven transgene expression in EnAd, but none increase virus replication. NMuMG-CD46 cells were seeded at 1 × 104 cells/well in a 96-well plate before coinfection with 5000 virus particles/cell of either (a) EnAd-CMV-GFP or (b) EnAd-SA-GFP and recombinant EnAd clones expressing individual MAV1 genes under the control of the CMV promoter or a combination of all tested ORFs (‘pool’). Five days post-infection, GFP expression in the cells was quantified by flow cytometry. Data represent biological triplicates, shown as mean ± SEM. Blue bars represent early viral transcriptional units; green, intermediate; yellow, late. c Significance within each treatment was assessed using one-way ANOVA with Dunnett’s Post Hoc analysis compared to infection with either EnAd-CMV-GFP or EnAd-SA-GFP alone (‘single’). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, not significant. Black asterisks represent a significantly higher levels compared to single infections; red, significantly lower levels. ORF, open reading frame; CMV, cytomegalovirus immediate-early promoter; MLP, adenovirus major late promoter

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