Fig. 2From: Influenza vaccination of cancer patients during PD-1 blockade induces serological protection but may raise the risk for immune-related adverse eventsInflammatory chemokines and lymphocytes in the peripheral blood upon influenza vaccination. (a-c) Measurement of chemokines before and after vaccination is shown. Chemokines were measured by a multiplex flow cytometry assay. CCL2 (a) CXCL10 (b) and CCL17 increased over time. (d) Measurement of percent of CD4 (d), CD8 (e) cells were performed by flow cytometry and T cells were defined by gating on living CD45 positive, CD3 positive lymphocytes. (f) Determination of naïve (CCR7 positive, CD45RA positive) and effector memory T cells (EM) in peripheral blood upon vaccination. *p < 0.05 by Student’s t test, ***p < 0.001 by Student’s t testBack to article page