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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Influenza vaccination of cancer patients during PD-1 blockade induces serological protection but may raise the risk for immune-related adverse events

Fig. 2

Inflammatory chemokines and lymphocytes in the peripheral blood upon influenza vaccination. (a-c) Measurement of chemokines before and after vaccination is shown. Chemokines were measured by a multiplex flow cytometry assay. CCL2 (a) CXCL10 (b) and CCL17 increased over time. (d) Measurement of percent of CD4 (d), CD8 (e) cells were performed by flow cytometry and T cells were defined by gating on living CD45 positive, CD3 positive lymphocytes. (f) Determination of naïve (CCR7 positive, CD45RA positive) and effector memory T cells (EM) in peripheral blood upon vaccination. *p < 0.05 by Student’s t test, ***p < 0.001 by Student’s t test

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