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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Co-transfer of tumor-specific effector and memory CD8+ T cells enhances the efficacy of adoptive melanoma immunotherapy in a mouse model

Fig. 2

Combinatorial ACT results in a stronger intratumoral T cell response. Seven days after tumor inoculation, Ly5.2+ mice were treated with 105 Teff, 105 Tmem, or 5 × 104 Teff + 5 × 104 Tmem derived from Ly5.1+ mice. TIL were harvested from melanoma tumors resected 4 or 14 days after ACT and stained for flow cytometric analysis. (a) Representative TIL data (gated on lymphocyte populations) demonstrate that CD8+ T cells comprised the highest percentage of TIL after effector ACT on day 4 and after combinatorial ACT on day 14. (b) Overall comparison of total numbers of intratumoral CD8+ T cells on days 4 and 14 demonstrated a transient increase after effector ACT and a sustained increase after memory ACT and combinatorial ACT. This experiment was repeated twice with similar results, 4–5 mice per group. (* p < 0.05 compared with control condition; † p < 0.05 compared with day 4)

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