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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Co-transfer of tumor-specific effector and memory CD8+ T cells enhances the efficacy of adoptive melanoma immunotherapy in a mouse model

Fig. 4

Combinatorial ACT results in the strongest level of systemic CD8+ T cell responsiveness to tumor antigen. Seven days after tumor inoculation, Ly5.2+ mice were treated with 105 Teff, 105 Tmem, or 5 × 104 Teff + 5 × 104 Tmem derived from Ly5.1+ mice. Spleens were resected 14 days after ACT and splenocytes were incubated with media alone (control) or with tumor antigen (GP33). (a) Representative data demonstrate negligible levels of IFNγ expression by splenocytes in response to GP33 stimulation after no ACT or effector ACT, with increasing levels seen after memory ACT and combinatorial ACT, respectively. (b) The highest levels of IFNγ expression by splenocytes in response to GP33 stimulation were seen after combinatorial ACT. This experiment was repeated with similar results, 4 mice per group. (* p < 0.05 compared with control condition; † p < 0.05 compared with effector ACT, ‡ p < 0.05 compared with memory ACT)

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