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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Enhanced susceptibility of cancer cells to oncolytic rhabdo-virotherapy by expression of Nodamura virus protein B2 as a suppressor of RNA interference

Fig. 1

B2 enhances VSV∆51 replication and alters miRNA levels in cancer cell lines. a M14 or 786-O cells were infected with VSVΔ51 virus and small-RNA deep sequencing was performed. Virus-derived small RNAs have a length bias towards 22-mers. The enrichment for 22-mers is indicated for positive strand vsRNAs. b Virus concentrations of supernatants collected from M14 or 786-O cells expressing fluorescently-tagged B2 or empty vector and infected with VSVΔ51 at an MOI of 0.1 for 24 h. NS: P > 0.1; *P < 0.1, **P < 0.01, ***P < 0.001, using Student’s t-test. Only significantly different pairs are indicated. c Schematic representation of the VSV∆51-B2 and VSV∆51-GFP viral backbones. d Western blot analysis of Vero cells infected at an MOI of 1 with VSVΔ51 or VSVΔ51-B2 for 24 h. The membranes were probed for VSV proteins, His-tagged B2 and GAPDH. e MiRNA levels from 786-O cells infected with VSVΔ51-GFP or VSVΔ51-B2 for 18 h as determined by qPCR. The results were normalized to mock uninfected levels as explained in the material and methods section. NS: P > 0.1, *P < 0.1, **P < 0.01, ***P < 0.001, using Student’s t-test. Only significantly different pairs are indicated on the figure

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