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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Enhanced susceptibility of cancer cells to oncolytic rhabdo-virotherapy by expression of Nodamura virus protein B2 as a suppressor of RNA interference

Fig. 3

VSV∆51-B2 modulates IFN response and cytokine production. a Microarray analysis of M14 cells infected with VSVΔ51-GFP or VSVΔ51-B2 at low and high MOI as indicated. b Enrichment of cytokine and cytokine activity in the microarray, associated with an IFN response. c qPCR analysis of IFN-β expression of 786-O cells infected for various times. IFN-β levels were normalized to GAPDH levels within each sample. d ELISA for IFN-β from supernatants of 786-O cells infected with VSVΔ51-GFP or VSVΔ51-B2 at an MOI of 0.1 for 24 h. e Virus outputs of VSVΔ51-GFP and VSVΔ51-B2 obtained from 786-O cells pre-treated with vaccinia Copenhagen virus conditioned-media. Virus-cleared supernatants from HeLa cells that were infected with vaccinia Copenhagen virus at an MOI of 1 for 48 h or left uninfected were transferred onto 786-O cells prior to infection with VSVΔ51-GFP or VSVΔ51-B2 for 48 h. NS: P > 0.1, *P < 0.1, **P < 0.01, ***P < 0.001, using Student’s t-test. Only significantly different pairs are indicated on the figure

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