Fig. 5
From: Dexamethasone-induced immunosuppression: mechanisms and implications for immunotherapy
CTLA-4 blockade enhances survival of dexamethasone-treated mice. a CTLA-4 was measured on circulating CD4 (left) and CD8 (right) T cells 1 h following oral gavage of vehicle or the indicated concentration of dexamethasone. Each cohort contained eight mice with intracranial GL261 tumors. Vehicle and dexamethasone-treated cohorts were statistically analyzed with an unpaired two-tailed student’s T-test. b Schema of treatment cohorts for (c-d). GL261 ffluc-mCherry glioma cells were orthotopically implanted into C57BL/6 mice one week before treatment initiation. Luminescence readings were acquired 6 days following tumor implantation and weekly thereafter. Mice were treated with vehicle or dexamethasone as indicated. CTLA-4 blocking antibody or isotype control were administered on days 13, 16, and 19 following tumor implantation. c Luminescence of tumor-bearing mice at days 13 and 20 following tumor implantation. d Kaplan Meier survival curves of mice receiving the indicated treatments. n = 7 to 8 mice per cohort. Data are representative of two independent experiments