Fig. 1
From: CART cells are prone to Fas- and DR5-mediated cell death
After their initial expansion, HER2-specific CART cells undergo apoptosis. a Schemes of tested HER2-CAR configurations. b Schematic representation of the experimental protocol c Frequencies of BFP or HER2-CART cells bearing various CAR co-stimulatory domains out of total live cells in the spleen at day 7 post rLm-OVA infection. d Representative FACS plots of CART cell frequencies of CART cells at day 5, 6 and 7 in the transferred OT-1 population. e Fas, FasL, DR5, TRAIL and Annexin V expression and viability staining in BFP or CAR-positive OT-1 T cells at day 6 and 7 post infection. f Scheme of the in vivo rescue of CART cells with recombinant Fas-Fc and DR5-Fc recombinant proteins. g Percentage of HER2- positive OT-1 T cells at day 7 post infection with rLm-OVA in the spleen and liver of mice that received either the control hIgG1 or both Fas-Fc and DR5-Fc. Statistical analysis by One-way ANOVA (c and e) and by T-test (g), n = 4 (c-e) or 5 (g) mice per group. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001