Fig. 2

mCALR multimer⁺ CD8⁺ T cell isolation (a) Pools of HLA-A*03:01 and HLA-B*07:02 multimers containing putative mCALR neoepitopes were used to enrich CD8⁺ T cells from HLA-A*03:01⁺ and HLA-B*07:02⁺ healthy donor PBMCs. (n = 3) (b) Following enrichment and expansion of mCALR multimer⁺ T cells, numerous mCALR multimer⁺ CD8⁺ T cell populations were detected by combinatorial-encoded multimer staining. c mCALR multimer⁺ CD8⁺ T cell clones were isolated by single-cell sorting and identified by single or dual-colour multimer staining