Fig. 4
From: Isolation of T cell receptors targeting recurrent neoantigens in hematological malignancies
mCALR TCR-engineered T cell recognition of mCALR-expressing target cells (a) mCALR TCR transduction efficiency was determined by mouse TCRβ (mTCRβ) staining and dual-colour mCALR multimer staining. Mock-transduced T cells were used to determine positive staining. (n = 3) (b) mCALR TCR-engineered T cells were co-cultured with LCLs loaded with decreasing concentrations of mCALR peptide at a 1:1 ratio and assessed for reactivity by IFN-γ intracellular cytokine staining. (n = 3) (c) mCALR TCR-transduced T cells were co-cultured with mCALR-expressing target cells at a 1:1 ratio and assessed for reactivity by IFN-γ intracellular cytokine staining. (n = 3)