Fig. 5

Isolation of additional putative neoepitope TCRs targeting recurrent mutations in hematological malignancies (a) Neoepitope-specific CD8⁺ T cells were enriched using neoepitope-bound multimers. After expansion of enriched cells, neoepitope multimer⁺ CD8⁺ T cell populations could be detected by combinatorial-encoded multimer staining. (n = 1) (b) Neoepitope multimer⁺ CD8⁺ T cells were cloned by single-cell sorting. Four FBXW7 TVH, four FBXW7 TVC and five p53 NQR CD8⁺ T cell clones were isolated. Representative multimer staining flow plots are shown with dual-colour multimer staining shown on the x and y axis. c Neoepitope T cell clones were assessed for reactivity against neoepitope peptide- or irrelevant peptide- loaded LCLs (10 μM) by CD137 staining. (n = 1)