Isolation of T cell receptors targeting recurrent neoantigens in hematological malignancies

Table 1 Putative HLA class I mCALR neoepitopes

Neoepitope	Peptide	Predicted HLA Restriction	Predicted HLA binding affinity (nM)
RPRTSCREA	CALR-REA	HLA-B*07:02	12
SPARPRTSC	CALR-SPA	HLA-B*07:02	22
RMRRTRRKM	CALR-RMR	HLA-B*07:02	56
RPRTSCREAC	CALR-REAC	HLA-B*07:02	23
RMMRTKMRMR	CALRp2	HLA-A*03:01	41
KMRMRRMRR	CALRp7	HLA-A*03:01	35
RTRRKMRRK	CALRp15	HLA-A*03:01	54

NetMHC was used to identify neoepitopes derived from the 36 amino acid mCALR C terminus that are predicted to bind strongly (< 500 nM) to common European Caucasoid HLA class I alleles