Skip to main content

Table 1 Primary antibody information for Multiplex IHC/IF panels

From: Multidimensional, quantitative assessment of PD-1/PD-L1 expression in patients with Merkel cell carcinoma and association with response to pembrolizumab

Position Antibody Clone (host)/Company Dilution Incubation (min) TSA dye
Panel 1
 1 PD-L1 SP142 (rabbit)/Spring Bio 1:800 60 620
 2 PD-1 EPR4877(2) (rabbit)/AbCam 1:1000 30 650
 3 NSE BBS/NC/VI-H14(mouse)/Dako 1:1000 60 570
 4 CD68 PGM-1(mouse)/Dako 1:500 30 540
 5 CD8 4B11(mouse)/AbD 1:100 30 520
 6 DAPI Perkin Elmer Opal 7-color kit 2 drops/ml 5 NA
Panel 2
 1 FoxP3 236A/E7(mouse)/abcam 1:100 30 570
 2 NSE BBS/NC/VI-H14(mouse)/Dako 1:400 30 620
 3 PD1 EPR4877(2)(rabbit)/abcam 1:500 120 650
 4 CD4 EP204(rabbit)/Sigma 1:50 120 540
 5 CD20 L26(mouse)/Leica 1:800 30 520
 6 CD8 4B11(mouse)/AbD 1:100 30 690
 7 DAPI Perkin Elmer Opal 7-color kit 2 drops/ml 5 NA