Table 1 Primary antibody information for Multiplex IHC/IF panels
Position | Antibody | Clone (host)/Company | Dilution | Incubation (min) | TSA dye |
|---|---|---|---|---|---|
Panel 1 | |||||
 1 | PD-L1 | SP142 (rabbit)/Spring Bio | 1:800 | 60 | 620 |
 2 | PD-1 | EPR4877(2) (rabbit)/AbCam | 1:1000 | 30 | 650 |
 3 | NSE | BBS/NC/VI-H14(mouse)/Dako | 1:1000 | 60 | 570 |
 4 | CD68 | PGM-1(mouse)/Dako | 1:500 | 30 | 540 |
 5 | CD8 | 4B11(mouse)/AbD | 1:100 | 30 | 520 |
 6 | DAPI | Perkin Elmer Opal 7-color kit | 2 drops/ml | 5 | NA |
Panel 2 | |||||
 1 | FoxP3 | 236A/E7(mouse)/abcam | 1:100 | 30 | 570 |
 2 | NSE | BBS/NC/VI-H14(mouse)/Dako | 1:400 | 30 | 620 |
 3 | PD1 | EPR4877(2)(rabbit)/abcam | 1:500 | 120 | 650 |
 4 | CD4 | EP204(rabbit)/Sigma | 1:50 | 120 | 540 |
 5 | CD20 | L26(mouse)/Leica | 1:800 | 30 | 520 |
 6 | CD8 | 4B11(mouse)/AbD | 1:100 | 30 | 690 |
 7 | DAPI | Perkin Elmer Opal 7-color kit | 2 drops/ml | 5 | NA |