Fig. 6

Ibuprofen enhances tumor draining lymph node T cell abundance and cytotoxic programming in mice. a Representative pseudo-colored composite of a T cell centric 7 antibody multiplex IHC image of tumor draining lymph node from an INV group mouse at 3wk post tumor injection (study end). CD45 is shown in red, CD3 in yellow, CD8 in green, Foxp3 in orange, GzmB in purple, Rorgt in light blue, and nuclei in blue. Quantitation of multiplex IHC signals of tumor draining lymph node to determine b CD3+ proportion of CD45+ cells, c CD3 + CD8+ and CD3 + CD4+ proportion of CD45+ cells, d Rorγt + proportion of CD8 + CD3 + CD45+ and CD4 + CD3 + CD45+ cells, and e Granzyme B (GzmB) + proportion of CD8 + CD3 + CD45+ and CD4 + CD3 + CD45+ cells. f Hierarchical clustering of lymph node multiplex IHC data show an association of evaluated parameters with tumor size and tumor cohort. Determination of statistically significant differences were based upon two-tailed Student’s t-test between the indicated groups with p < 0.05 (*) and p < 0.01 (**)