Fig. 4

HCC clearance by IL-12-LNP is associated with the recruitment of activated immune cells. a-b Immunohistochemistry for CD3+ pan T cells in liver tumor (a), and surrounding normal liver (b) tissues isolated from control (NST-LNP, n = 6), and IL-12 treated (IL-12-LNP, n = 5) MYC-driven HCC mice. c-d Immunohistochemistry for CD4+ immune cells (helper T, macrophages and DCs) in the liver tumor (c), and surrounding normal liver (d) tissues isolated from control (NST-LNP, n = 6), and IL-12 treated (IL-12-LNP, n = 5) MYC-driven HCC mice. e-f Immunohistochemistry for activated CD44+ T cells in liver tumor (e), and surrounding normal liver (f) tissues isolated from control (NST-LNP, n = 6), and IL-12 treated (IL-12-LNP, n = 5) MYC-driven HCC mice. Quantification for percent area positive of intratumoral CD3+ (a), CD4+ (c), and CD44+ (e) immune cells is provided to the right of the corresponding IHC images. Quantification is an average of 5 representative IHC images for each group. Red arrowheads within IHC images indicate positive staining for the specific types of immune cells measured. One representative image is shown from each group of mice. Scale bars = 50 μm. P-values were calculated using Student’s t-test. P values: **** p < 0.0001