Fig. 5

CD8+ T-cells that co-express PD-1 and CD137 express IFN-γ. Liver metastatic KPC tumor-bearing mice were sacrificed on day 14 after receiving treatment, and flow cytometry analysis was performed on the isolated tumor infiltrating CD8+ T-cells after incubation with CD3/CD28 stimulation beads. a IFN-γ production of CD8 + PD-1 + CD137+ T-cells. b IFN-γ production of CD8 + PD-1 + CD137+ and CD8 + PD-1 + CD137- T-cells in the GVAX + αPD-1 + Pre/Post-αCSF-1R group. N = 3–4 for each treatment group, and each data point represents cells from one mouse. The error bars represent mean with standard deviation. *** p < 0.001; NS, non-significant