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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Purinergic targeting enhances immunotherapy of CD73+ solid tumors with piggyBac-engineered chimeric antigen receptor natural killer cells

Fig. 3

Gene transfer process and NKG2D-CAR expression. a Diagram of the gene transfer process with PBAE 447/piggyBac-NKG2D.CAR complex. b and c Mean fluorescence intensity (MFI) of NKG2D expression. d Viability of NK cells after transfection with piggyBac-NKG2D.CAR/PBAE 447 as determined by CCK-8 assay. Data are presented as the mean ± SEM (n = 4). *P < 0.05

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