Fig. 3

Impact of mature DCs on the frequency and cytotoxicity of CD8⁺ T cells and NK cells in HGSC. a Representative images of CD8 immunostaining. Scale bar = 50 μm. b Density of CD8⁺ T cells in the tumor stroma and tumor nest in DC-LAMP^Lo versus DC-LAMP^Hi HGSCs (n = 81). Box plots: lower quartile, median, upper quartile; whiskers, minimum, maximum. c Percentage of IFN-γ⁺, IFN-γ⁺/GZMB⁺ and IFN-γ⁺/CD107a⁺ cells among CD8⁺CD3⁺ T cells from the HGSC of 10 DC-LAMP^Lo and 10 DC-LAMP^Hi patients after non-specific stimulation. Box plots: lower quartile, median, upper quartile; whiskers, minimum, maximum. d, e OS of HGSC patients who did not receive neoadjuvant chemotherapy, upon stratification based on median density of CD8⁺ cells alone (d) or plus DC-LAMP⁺ cells (e). Survival curves were estimated by the Kaplan-Meier method, and differences between groups were evaluated using log-rank test. Number of patients at risk are reported. f Representative images of NKp46 immunostaining. Scale bar = 50 μm. g Density of NKp46⁺ cells in the tumor stroma and tumor nest in DC-LAMP^Lo versus DC-LAMP^Hi HGSCs (n = 81). Box plots: lower quartile, median, upper quartile; whiskers, minimum, maximum. h Percentage of IFN-γ⁺, IFN-γ⁺/GZMB⁺ and IFN-γ⁺/PRF1⁺ cells among CD3⁻CD56⁺ NK cells from the HGSCs of 10 DC-LAMP^Lo and 10 DC-LAMP^Hi patients after non-specific stimulation. Box plots: lower quartile, median, upper quartile; whiskers, minimum, maximum. i OS of HGSC patients who did not receive neoadjuvant chemotherapy, upon stratification based on median density of DC-LAMP⁺ cells plus NKp46⁺ cells. Survival curves were estimated by the Kaplan-Meier method, and differences between groups were evaluated using log-rank test. Number of patients at risk are reported