Fig. 2

TIM-1⁺B cells are a regulatory B cell subset that exhibits a unique phenotype and function. a Flow cytometry analysis of the phenotypic characteristics of the TIM-1⁺B cells from the paired blood, liver tissue and HCC tissue samples (n = 10). b Analysis of the representative markers expressed by tumor-infiltrating TIM-1⁺ and TIM-1⁻B cells. c Flow cytometry analysis of the TNF-α and IFN-γ production of the tumor-infiltrating CD8⁺ effector T cells cocultured with tumor-infiltrating TIM-1⁺ B cells or TIM-1⁻ B cells. d Flow cytometry analysis of the TNF-α and IFN-γ production of CD8⁺ effector T cells in the tumor-infiltrating lymphocytes (TILs) and TILs without TIM-1⁺B cells groups. c-d The data are represented as the mean ± s.e.m. of three independent experiments. e Associations between tumor-infiltrating IFN-γ⁺CD8⁺ T cells and tumor-infiltrating TIM-1⁺B cells (n = 9). *P < 0.05, **P < 0.01, and ***P < 0.001