Fig. 3
TIM-1+B cells produce IL-10 when TIM-1 interacts with its ligand. a Tumor-infiltrating TIM-1− and TIM-1+ B cells (1 × 105 cells/well) were purified by flow sorting and cultured in the presence of an anti-TIM-1 antibody (2 μg/ml) or PBS as control in vitro for 72 h. The supernatants from the cultures were collected, and the production of IL-10 was determined by ELISA (n = 3). b The flow cytometry analysis of TIM-4 expression on CD11b+ myeloid cells from the paired tumor tissue, liver tissue and blood samples (n = 14) is shown. c Representative immunofluorescence images of CD20 (red), TIM-4 (green) and nuclear staining with DAPI (blue) in HCC tissue are shown. **P < 0.01