Fig. 2
TRAPs exposed macrophages inhibit T cell proliferation via PD-L1 and IL-10. a-d Representative determination of T cell proliferation by flow cytometry. a, b, d CFSE-labeled purified CD3+ T cells were activated by immobilized anti-CD3 plus soluble anti-CD28 mAb, and were either cultured alone or cocultured with BMDMs pretreated with or without TRAPs at a ratio of 5:1 for 3 d. b A transwell chamber was used to separate BMDMs from T cells. d BMDMs were cocultured with T cells in the presence of anti-PD-L1 mAb, anti-IL-10 mAb or control IgG. c BMDMs were left untreated or pretreated with 10 μg/ml B16F10 TRAPs or B16F10-OVA TRAPs for 2 d, and loaded with OVA257–264 peptide SIINFEKL (1 μg/ml) for 2 h, washed, were then incubated with CFSE-labeled OT-I splenocytes for 3 d at a ratio of 1:20. Data are representative of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 by unpaired t test (a-d)