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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Tumor cell-released autophagosomes (TRAPs) promote immunosuppression through induction of M2-like macrophages with increased expression of PD-L1

Fig. 3

Immunosuppression mediated by TRAPs treated BMDMs is dependent on TLR4-MyD88 signaling. a Expression analysis of PD-L1 by flow cytometry. BMDMs derived from WT, TLR2−/−, TLR4−/−, or MyD88−/− mice were incubated with TRAPs (10 μg/ml) for 48 h. b ELISA detection of IL-10 secreted from BMDMs exposed to TRAPs (10 μg/ml) for 72 h. c, d Detection of OT-I CD8+ T cell division by flow cytometry. BMDMs derived from WT, TLR2−/−, TLR4−/−, or MyD88−/− mice were left untreated or pretreated with B16F10 TRAPs (10 μg/ml) for 2 d, and pulsed with peptide SIINFEKL (1 μg/ml) for 2 h, washed, and followed by coculture with CFSE-labeled OT-I splenocytes for 3 d at a ratio of 1:20. Data (mean ± SEM) are representative of three independent experiments. **p < 0.01, ***p < 0.001 by unpaired t test. (b and d)

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