Fig. 4

Both diffuse and nodular tumors were induced in C57BL/6j mice by HDI with pKT2/CLP-AKT-Ags-LUC, pT/Caggs-NRASV12 and pCMV(CAT)T7- SB100. a Bioluminescence of mice at indicated time points post HDI. Two different patterns, rapid and slow progression, of bioluminescence activity were shown. *The humane endpoint for tumor-bearing mice. b Representative livers harvested from the mice with rapid progression (d28) or slow progression (d70) after HDI. H&E staining of c the liver section from a representative mouse developing diffuse liver tumors at day 28 post HDI and d the liver section from one representative mouse developing nodular liver tumors at day 70 post HDI. T: tumor region, L: adjacent liver tissue; Scale bars, 1000 or 100 μm. e The correlation of the size of nodular tumors and their bioluminescence by IVIS (n = 29). f Staining of lipid, Ki-67, F4/80, Gr-1, α-SMA, CD31, CD4, CD8, CD19 of the diffuse and nodular tumor tissues were shown. Scale bars, 100 or 20 μm